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Optimization of BE-91 Strain Xylanase Activity Determination Conditions

Xia Zheng

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Abstract

Conditions for the activity determination of xylanase from BE91 strain were optimized by one factor experimentation and orthogonal test. The results showed that the reaction between 100 times diluted xylanase and 0.8% xylan was carried out at the ratio of 1∶9 (V / V) in pH 5.4～5.6, 0.1 mol/ L citric acid sodium citrate buffer for 3 min at 62℃, after adding 2.0 mL DNS to color for 10 min in boiling water bath, the best result was obtained at 540 nm. Under the optimum conditions, the xylanase activity was up to 350.24 U / mL.

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What this paper is about

Conditions for the activity determination of xylanase from BE91 strain were optimized by one factor experimentation and orthogonal test. The results showed that the reaction between 100 times diluted xylanase and 0.8% xylan was carried out at the ratio of 1∶9 (V / V) in pH 5.4～5.6, 0.1 mol/ L citric acid sodium citrate buffer for 3 min at 62℃, after adding 2.0 mL DNS to color for 10 min in boiling water bath, the best result was obtained at 540 nm. Under the optimum conditions, the xylanase activity was up to 350.24 U / mL.

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Available abstract

Conditions for the activity determination of xylanase from BE91 strain were optimized by one factor experimentation and orthogonal test. The results showed that the reaction between 100 times diluted xylanase and 0.8% xylan was carried out at the ratio of 1∶9 (V / V) in pH 5.4～5.6, 0.1 mol/ L citric acid sodium citrate buffer for 3 min at 62℃, after adding 2.0 mL DNS to color for 10 min in boiling water bath, the best result was obtained at 540 nm. Under the optimum conditions, the xylanase activity was up to 350.24 U / mL.

Key concepts: Xylanase, Citric acid, Chemistry, Boiling, Sodium citrate, Xylan, Strain (injury), Chromatography

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