2004•Acta Agriculturae ZhejiangensisRequires access

Studies on the method of measuring the enzyme activity of feed xylanase

Chao Yuan

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Abstract

This study discussed various factors of affecting xylanase activity and a measuring method of feed xylanase by the DNS. The results showed that the absorbance of the hydrolysis products was at the wave length of 540-550 nm with the optimum conditions of reaction temperature of 40℃,pH of 5.0,concentration of substrate of 1%, reaction time of 10 min,and heating time of 5 min.

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What this paper is about

This study discussed various factors of affecting xylanase activity and a measuring method of feed xylanase by the DNS. The results showed that the absorbance of the hydrolysis products was at the wave length of 540-550 nm with the optimum conditions of reaction temperature of 40℃,pH of 5.0,concentration of substrate of 1%, reaction time of 10 min,and heating time of 5 min.

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Available abstract

This study discussed various factors of affecting xylanase activity and a measuring method of feed xylanase by the DNS. The results showed that the absorbance of the hydrolysis products was at the wave length of 540-550 nm with the optimum conditions of reaction temperature of 40℃,pH of 5.0,concentration of substrate of 1%, reaction time of 10 min,and heating time of 5 min.

Key concepts: Xylanase, Absorbance, Hydrolysis, Chemistry, Substrate (aquarium), Enzyme, Chromatography, Food science

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