Statistical optimization of activity and stability of β-xylanase produced by newly isolated Thermomyces lanuginosus THKU-49 using central composite design

A factorial design was performed to find the best conditions of pH and temperature for #946-xylanase activity and to maintain its activity for prolonged periods of time of pure xylanase produced by newly isolated Thermomyces lanuginosus THKU-49. The central composite design (CCD) used for the analysis of treatment combinations showed that a regression models of optimization of xylanase activity and xylanase stability were good agreement to experimental results with R2 = 0.98 and 0.99, respectively. The maximum activity of xylanase was obtained at 66 o C and pH 6.3. The temperature and pH for maximum enzyme stability was 70 o C and pH 7.3, respectively. Under this condition xylanase having half-life of 825 min indicated the highest thermostable xylanase.