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Studies on Tissue Culture Technique of Cymbidium wenshanense

LI Zhi-lin

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Abstract

Using seeds as explants,tissue culture technique of C.wenshanense including sterile seed germination,regeneration and rooting was experimented and studied.The experiment results showed that the sterile seed germination medium was 1/2 MS+0.2 mg/L BA+0.2 mg/L NAA,the medium for regeneration was 1/2 MS + 1.5 mg/L BA+0.2 mg/L NAA+80 g/L mashed banana+1 g/L Huabao No.4,the medium for root inducing was 1/2 MS+0.6 mg/L NAA+0.2 mg/L BA+0.3 % AC.The tissue culture plants of 6 to 8 cm high could be transplanted.

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What this paper is about

Using seeds as explants,tissue culture technique of C.wenshanense including sterile seed germination,regeneration and rooting was experimented and studied.The experiment results showed that the sterile seed germination medium was 1/2 MS+0.2 mg/L BA+0.2 mg/L NAA,the medium for regeneration was 1/2 MS + 1.5 mg/L BA+0.2 mg/L NAA+80 g/L mashed banana+1 g/L Huabao No.4,the medium for root inducing was 1/2 MS+0.6 mg/L NAA+0.2 mg/L BA+0.3 % AC.The tissue culture plants of 6 to 8 cm high could be transplanted.

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Available abstract

Using seeds as explants,tissue culture technique of C.wenshanense including sterile seed germination,regeneration and rooting was experimented and studied.The experiment results showed that the sterile seed germination medium was 1/2 MS+0.2 mg/L BA+0.2 mg/L NAA,the medium for regeneration was 1/2 MS + 1.5 mg/L BA+0.2 mg/L NAA+80 g/L mashed banana+1 g/L Huabao No.4,the medium for root inducing was 1/2 MS+0.6 mg/L NAA+0.2 mg/L BA+0.3 % AC.The tissue culture plants of 6 to 8 cm high could be transplanted.

Key concepts: Tissue culture, Germination, Explant culture, Botany, Biology, Murashige and Skoog medium, Horticulture, Sterile water

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