2001Xibei zhiwu xuebaoRequires access

Studies of tissue culture techniques of Hippophae L.

Liang Zong

Open publisher page 2 citations

Abstract

The sterile plants and meristem of germinated dormancy buds of Hippophae rhamnoides L.were taken as explants cultured \%in vitro\% to induce the plant regeneration.The basic culture medium is 1/4 MS medium.The results showed that the shoot differentiation medium is 1/4 MS+6 BA 0.30 mg/L+NAA 0.002 mg/L,the multiplication medium is 1/4 MS+6 BA 0.1 mg/L+NAA 0.004 mg/L,The medium for rooting is 1/4 MS+NAA 0.05 mg/L+IBA 0.2 mg/L and the callus inducing medium is 1/4 MS+2,4 D 0.3 mg/L.

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What this paper is about

The sterile plants and meristem of germinated dormancy buds of Hippophae rhamnoides L.were taken as explants cultured \%in vitro\% to induce the plant regeneration.The basic culture medium is 1/4 MS medium.The results showed that the shoot differentiation medium is 1/4 MS+6 BA 0.30 mg/L+NAA 0.002 mg/L,the multiplication medium is 1/4 MS+6 BA 0.1 mg/L+NAA 0.004 mg/L,The medium for rooting is 1/4 MS+NAA 0.05 mg/L+IBA 0.2 mg/L and the callus inducing medium is 1/4 MS+2,4 D 0.3 mg/L.

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Available abstract

The sterile plants and meristem of germinated dormancy buds of Hippophae rhamnoides L.were taken as explants cultured \%in vitro\% to induce the plant regeneration.The basic culture medium is 1/4 MS medium.The results showed that the shoot differentiation medium is 1/4 MS+6 BA 0.30 mg/L+NAA 0.002 mg/L,the multiplication medium is 1/4 MS+6 BA 0.1 mg/L+NAA 0.004 mg/L,The medium for rooting is 1/4 MS+NAA 0.05 mg/L+IBA 0.2 mg/L and the callus inducing medium is 1/4 MS+2,4 D 0.3 mg/L.

Key concepts: Explant culture, Tissue culture, Callus, Murashige and Skoog medium, Shoot, Hippophae rhamnoides, Germination, Botany

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