Tissue Culture Experiment on Different Loquat Explants

[Objective] The aim was to lay a foundation for shortening the crossing-breeding period of loquat and breeding new loquat cultivar by transgenic technology.[Method] With seeds,shoot-tips and tender stem-segments of Guanyu loquat as explants,the sterile seedlings were studied.[Result] The germination rate of seeds,which could be up to 100%,was high.The leaves from shoot-tips expanded best on the medium MS + 6-BA 1.2 mg/L + NAA 0.6 mg/L + GA3 0.5 mg/L.The stem-segment germinated best on the medium MS + 6-BA 1.5 mg/L + NAA 0.3 mg/L + GA3 0.5 mg/L,its germination rate reached 68.9% and that was secondary on the medium MS + 6-BA 1.2 mg/L + NAA 0.4 mg/L + GA3 0.5 mg/L.The effect of inducing adventitious bud from stem-segment was best on the medium MS + 6-BA 1.5 mg/L + NAA 0.3 mg/L + GA3 0.5 mg/L and that was secondary on the medium MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L + GA3 0.2 mg/L.The proliferation effect of initial sterile seedlings was best on the medium MS + 6-BA 1.5 mg/L + NAA 0.3 mg/L.[Conclusion] The allocation of medium components had very great influences on the germination and proliferation of explants,especially for hormone concn.