Detection and drug resistance of extended spectrum β-lactamase-producing bacteria

OBJECTIVE To explore the detection rate and drug resistance of extended spectrum β-lactamase(ESBLs) producing Escherichia coli and Klebsiella pneumoniae so as to guide the clinical treatment.METHODS Bacterial identification,drug susceptibility testing(MIC method) and ESBLs detection were performed with French Merieux VITEK-2 Compact automated bacterial culture and identification device,statistical analysis was performed with Whonet 5.3 software.RESULTS E.coli isolates were mainly from urine,accounting for 59.8%,K.pneumoniae isolates were mainly from sputum,accounting for 73.0%,Acid-producing Klebsiella isolates were mainly from sputum,accounting for 75.0%;of 714 strains of pathogens,there were 220 strains of ESBLs-producing bacteria isolated with the detection rate of 30.8%,among which there were 174 strains of ESBLs-producing E.coli with the detection rate of 35.3%,189 strains of ESBLs-producing K.pneumoniae with the detection rate of 19.0%,and 32 strains of ESBLs-producing Acid-producing Klebsiella with the detection rate of 31.2%;the drug resistance rates of ESBLs-producing E.coli and K.pneumoniae to imipenem was 0,the resistance rates to piperacillin/tazobactam sodium were less than 4.2%;the resistance rates of ESBLs-producing strains were significantly higher than non-ESBLs-producing strains to all of the antibiotics except imipenem,the differences were statistically significant antimicrobial drug was significantly higher than non-ESBLs producing strains,the difference was statistically significant(P0.05).CONCLUSION Detection rate of ESBLs-producing bacteria and drug resistance keep an upward tendency and we should strengthen the monitoring of bacterial resistance.