Simultaneous determination of ferulic,protocatechuic acid,and cinnamaldehyde in Jiefeng Wine by HPLC

AIM To establish an HPLC method for simultaneously determining ferulic,protocatechuic acid,and cinnamaldehyde in Jiefeng Wine(Angelicae Sinensis Radix,Cibotii Rhizoma,Cinnamomi Ramulus,etc.).METHODS The column C18 was selected.The mobile phase was gradient elution(0-35 min,75%B-55%B),with acetonitrile(A)-0.1% glacial acetic acid(B).The flow rate was 1.0 mL/min,the column temperature was maintained at 30 ℃,and the detection wavelength was set at 260 nm.RESULTS The results showed a good relationship in the ranges of 0.024 26-0.242 6 μg for ferulic,0.025 02-0.250 2 μg for protocatechuic acid,0.010 48-0.104 8 μg for cinnamaldehyde(r=0.999 9),and the average recoveries were 98.2%,99.9% and 99.2%,respectively.Their RSDs were 1.52%,0.78% and 1.14%,respectively.CONCLUSION The method is simple,accurate and reproducible,and can be used for the quality control of Jiefeng Wine.