Simultaneous Determination of Hesperidin, Ferulic Acid, Cinnamic Acid and Cinnamaldehyde in Chinese Tonic Wine by High Performance Liquid Chromatography

Abstract This study aims to establish a HPLC method for the simultaneous determination of hesperidin (HE), ferulic acid (FA), cinnamic acid (CA) and cinnamaldehyde (CAD) in a Chinese tonic wine, Ma‐Zu‐Wan‐Show‐Yao‐Jyo. After filtering the tonic wine, the filtrate was separated on a C18 column (250 × 4.6 mm, 5 μm) by gradient elution of acetonitrile‐aqueous solution. The regression equations were linear (r ≥ 0.9999) in the range of 1.5‐150.0 μg/mL for HE, 0.3‐60.0 μg/mL for FA, 0.06‐6.0 μg/mL for CA, and 0.1‐80.0 μg/mL for CAD. The relative standard deviation and relative error were less than 5.0% and 4.0%, respectively, for intra‐ and inter‐day analyses. The limits of detection (LOD) were 0.6, 0.075, 0.012 and 0.02 μg/mL for HE, FA, CA and CAD (S/N = 3, 20 μ1 injection) respectively. This method can be used to standardize the quality control of Chinese tonic wine to ensure accuracy and efficiency in the future.