Simultaneous Determination of Ferulic acid and Cinnamaldehyde in Dangguijianzhong Decoction by HPLC

Objective:To establish a HPLC method for determining simultaneously the contents of ferulic acid and cinnamaldehyde in Danggui-jianzhong decoction.Methods:Dikma C18column(4.6mm×250mm,5μm)was used with a step gradient of Acetonitrile-0.5% Acetic acid at a flow rate of 1.0mL·min-1.The detection wavelength was set at 325 nm,and the column temperature was at 25℃.Results:There was good linear relationship in the range of 3.684~36.84 mg·L-1for ferulic acid and 38.56~385.6mg·L-1for cinnamaldehyde.The correlation coefficinets of ferulic acid and cinnamaldehyde were higher than 0.999.The average recovery of ferulic acid was 100.47%(n=6),and cinnamaldehyde was 100.52%(n=6).RSD of the above two components were 1.54%,1.75%,respectively.Conclusions:The method is simple,accurate and can be used as the method for quality assessment of Dangguijianzhong Decoction.