Study on liquid fermentation of mushroom mycelium

Using wheat bran extract as fermentation medium,the effect of fermentation medium on the extracellular polysaccharide and mycelium biomass was studied.The results shown that the optimum medium formula was as follows: wheat bran 60%,sucrose 1%,MgSO4 0.15%,KH2PO4 0.2%,and the optimum inoculum 12%.Variation of mycelium biomass,pH value,extracellular polysaccharide content during fermentation process was studied,as well.The optimal fermentation time was 11d,inoculum size was 12% and final pH value of fermentation broth was about 4.2.