Optimization of submerged fermentation conditions of Inonotus obliquus

Using biomass and polysaccharide yield as indicators,effects of different factors,such as carbon sources,nitrogen sources,inorganic salts,temperature,initial pH value and inoculation amount,on submerged fermentation of Inonotus obliquus were studied.Single-factor experimental design was used for optimum fermentation conditions.The optimum fermentation medium for polysaccharide production was obtained as follows: sucrose 1.8%,wheat bran 0.2%,KH2PO4 0.1% and MgSO4·7H2O 0.05% and optimum fermentation conditions were initial pH value 8.5,inoculation amount 10%(v/v) and fermentation temperature 25℃.