2010•China BrewingRequires access
Optimization of medium and fermentation conditions of Agaricus bisporus AS2796 in liquid culture
Liang Huang
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Abstract
Composition of medium and fermentation conditions of Agaricus bisporus AS2796 in liquid culture were optimized with one factor and orthogonal test methods.The results showed that the optimum medium was(g/L):sucrose 15.0,corn starch 30.0,peptone 2.0,soybean powder 30.0,MgSO4·7H2O 1.0,KH2PO4 1.5 and the optimum fermentation condition were initial pH value 6.5,fermentation under 25℃ for 7d,rotation speed 180r/min with broth volume 50ml,inoculum 5%.