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2006•Jiangsu Pharmaceutical and Clinical ResearchRequires access

Determination of Total Flavonoids in Polygonum cuspidatum from Different Habitats

Yi Chen, Taijun Hang, Xinxin Xu

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Abstract

OBJECTIVE To determine the total flavonoids in Polygonum cuspidatum from different habitats.METHODS Total flavonoids in Polygonum cuspidatum were extracted with 70% ethanol solution by ultrasonication.Total flavonoids were determined by colorimetry at 500 nm with aluminum nitrate as the coloring reagent at the presence of sodium nitrite and sodium hydroxide solution.The average recovery was 100.3% with RSD 1.89%(n=9).RESULTS The total flavonoids in Polygonum cuspidatum from Nanjing,Xuzhou,Guanxian,Yibing,Aba,Yulin,Nanchang,Shenzhen,Foshan,Yangjiang,Guangzhou,Luoyang,Anqing,Guilin,Nanning,Kunming,Wuhan,Changchun,Lianyuan were 10.98%,10.10%,9.60%,10.01%,9.14%,10.94%,8.92%,9.32%,12.34%,11.99%,7.19%,10.21%,16.73%,9.06%,9.29%,12.44%,13.95%,7.34% and 14.10%,respectively.CONCLUSION The colorimetric method was suitable for the determination of flavonoids in Polygonum cuspidatum.The total flavonoids content in the drug are different.

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What this paper is about

OBJECTIVE To determine the total flavonoids in Polygonum cuspidatum from different habitats.METHODS Total flavonoids in Polygonum cuspidatum were extracted with 70% ethanol solution by ultrasonication.Total flavonoids were determined by colorimetry at 500 nm with aluminum nitrate as the coloring reagent at the presence of sodium nitrite and sodium hydroxide solution.The average recovery was 100.3% with RSD 1.89%(n=9).RESULTS The total flavonoids in Polygonum cuspidatum from Nanjing,Xuzhou,Guanxian,Yibing,Aba,Yulin,Nanchang,Shenzhen,Foshan,Yangjiang,Guangzhou,Luoyang,Anqing,Guilin,Nanning,Kunming,Wuhan,Changchun,Lianyuan were 10.98%,10.10%,9.60%,10.01%,9.14%,10.94%,8.92%,9.32%,12.34%,11.99%,7.19%,10.21%,16.73%,9.06%,9.29%,12.44%,13.95%,7.34% and 14.10%,respectively.CONCLUSION The colorimetric method was suitable for the determination of flavonoids in Polygonum cuspidatum.The total flavonoids content in the drug are different.

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Available abstract

OBJECTIVE To determine the total flavonoids in Polygonum cuspidatum from different habitats.METHODS Total flavonoids in Polygonum cuspidatum were extracted with 70% ethanol solution by ultrasonication.Total flavonoids were determined by colorimetry at 500 nm with aluminum nitrate as the coloring reagent at the presence of sodium nitrite and sodium hydroxide solution.The average recovery was 100.3% with RSD 1.89%(n=9).RESULTS The total flavonoids in Polygonum cuspidatum from Nanjing,Xuzhou,Guanxian,Yibing,Aba,Yulin,Nanchang,Shenzhen,Foshan,Yangjiang,Guangzhou,Luoyang,Anqing,Guilin,Nanning,Kunming,Wuhan,Changchun,Lianyuan were 10.98%,10.10%,9.60%,10.01%,9.14%,10.94%,8.92%,9.32%,12.34%,11.99%,7.19%,10.21%,16.73%,9.06%,9.29%,12.44%,13.95%,7.34% and 14.10%,respectively.CONCLUSION The colorimetric method was suitable for the determination of flavonoids in Polygonum cuspidatum.The total flavonoids content in the drug are different.

Key concepts: Chemistry, Polygonum, Colorimetry, Chromatography, Food science, Traditional medicine, Medicine

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