Contents of total and free flavonoids in different parts of Mosla chinensis from different habitats

AIM To determine the contents of total and free flavonoids from different tissues of Mosla chinensis in different habitats,and to select and find the raw materials with higher contents and main storage sites of the flavonoids.METHODS Total flavonoids in Mosla chinensis were determined by UV spectrophotometer,and the optimized analysis conditions consisted of concentration of aluminum chloride(0.05 mol/L),pH 5.0 of acetate buffer with water bath at 50 ℃ and 30 min in detection wavelength of 420 nm.Free flavonoids(luteolin,apigenin and negletein) were measured by RP-HPLC with Welchrom TMC18(200 mm×4.6 mm,5 μm) column and mobile phase(0.4% phosphoric acid-methanol) in gradient elution mode at 1.0 mL/min flow velocity,350 nm detection wavelength and 25 ℃column temperature.RESULTS The results showed that the contents of luteolin,apigenin and negletein were good linearity and the correlation coefficients(r0.999) in 0.88-176 μg/mL,1.1-219.2 μg/mL and 1.55-149.4 μg/mL,respectively.The recoveries were 98.80%-102.01% with less than 5% RSDs,and the higher contents(5 955.35 μg/g and 4 362.28 μg/g) were respectively presented in the samples from Jiangxi and Hubei Province.Particularly,the highest contents(13 335.99 μg/g and 12 936.46 μg/g) were determined in the bud part of Mosla chinensis from above two provinces.CONCLUSION The study shows that the contents of total flavonoids in Mosla chinensis from different habitats are Jiangxi,Hubei,Henan,Zhejiang Province in descending order,and in different parts are flower bud,branchlet,stem,root in descending order.The contents of total flavonoids and mainly three free flavonoids in Mosla chinensis from Jiangxi Province are higher than those from other provinces,and they are mainly enriched in above parts of stem.