2005•The Chinese Journal of Modern Applied PharmacyRequires access
Determination of ursolic acid and oleanolic acid in Sambucus chinensis by RP-HPLC
Zou Sheng-qin
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Abstract
OBJECTIVE To develop a new method for determination of ursolic acid and oleanolic acid in Sambucus chinensis Lindl. METHODS The Symmetry C_(18)(3.9×150 mm,5 μm)was used as analytical colomn with methanol-water(88∶12)as mobile phase. The detection wavelength was at 220nm. The flow rate was 0.6 mL/min and the column temperature was 25℃. RESULTS The calibration curve of ursolic acid was linear over the range of 0.098~2.35μg, the correlation coefficient was 0.9999. The calibration curve of oleanolic acid was linear over the range of 0.099~2.38μg, the correlation coefficient was 0.9997.The average recoveries of ursolic acid and oleanolic acid were 97.9 % (RSD=2.11%) and 98.6 % (RSD=1.84%),respectively. CONCLUTION The content of ursolic acid and oleanolic acid can be simultaneously determined by HPLC.The method is accurate,sensitive and reliable, it can be used for quality control of medicinal materials of Sambucus chinensis Lindl.