ScholarLens Back to search
2005•Yaowu fenxi zazhiRequires access

RP-HPLC Determination of Oleanolic Acid and Ursolic Acid in Sambucus chinensis Lindl.

Wu Xiao

Open publisher page 1 citations

Abstract

Objective:To establish an RP - HPLC method for the determination of oleanolic acid and ursolic acid in Sambucus chinensis Undl.. Methods:A Hypersile -ODS2column (4. 6 mm 250 mm,5μm) was used with the mobile phase being methanol -water (85:15) ,flow rate being 0.8 mL·min-1 .determinating wavelength being 210 nm,the column temperature being 30℃. Results:The calibration curve was linear in the range of 2. 6 - 104.0 μg·mL-1 for oleanolic acid (r=0.9999) ,and the regression equation being Y=1.269×10~4X-8.296×10~3. The average recovery was 102.1%. The calibration curve was linear in the range of 2.3- 92.0μg·mL-1 for ursolic acid ( r = 0.9999) ,and the regression equation being Y=1.226×10~4X-5. 186×10~3. The average recovery was 97.6%. Conclusion:The method is simple, accurate and reproducible for quality control of Sambucus chinensis Lindl. .

About this research paper

What this paper is about

Objective:To establish an RP - HPLC method for the determination of oleanolic acid and ursolic acid in Sambucus chinensis Undl.. Methods:A Hypersile -ODS2column (4. 6 mm 250 mm,5μm) was used with the mobile phase being methanol -water (85:15) ,flow rate being 0.8 mL·min-1 .determinating wavelength being 210 nm,the column temperature being 30℃. Results:The calibration curve was linear in the range of 2. 6 - 104.0 μg·mL-1 for oleanolic acid (r=0.9999) ,and the regression equation being Y=1.269×10~4X-8.296×10~3. The average recovery was 102.1%. The calibration curve was linear in the range of 2.3- 92.0μg·mL-1 for ursolic acid ( r = 0.9999) ,and the regression equation being Y=1.226×10~4X-5. 186×10~3. The average recovery was 97.6%. Conclusion:The method is simple, accurate and reproducible for quality control of Sambucus chinensis Lindl. .

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To establish an RP - HPLC method for the determination of oleanolic acid and ursolic acid in Sambucus chinensis Undl.. Methods:A Hypersile -ODS2column (4. 6 mm 250 mm,5μm) was used with the mobile phase being methanol -water (85:15) ,flow rate being 0.8 mL·min-1 .determinating wavelength being 210 nm,the column temperature being 30℃. Results:The calibration curve was linear in the range of 2. 6 - 104.0 μg·mL-1 for oleanolic acid (r=0.9999) ,and the regression equation being Y=1.269×10~4X-8.296×10~3. The average recovery was 102.1%. The calibration curve was linear in the range of 2.3- 92.0μg·mL-1 for ursolic acid ( r = 0.9999) ,and the regression equation being Y=1.226×10~4X-5. 186×10~3. The average recovery was 97.6%. Conclusion:The method is simple, accurate and reproducible for quality control of Sambucus chinensis Lindl. .

Key concepts: Ursolic acid, Chemistry, Oleanolic acid, Chromatography, Calibration curve, High-performance liquid chromatography, Methanol, Detection limit

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
RP-HPLC Determination of Oleanolic Acid and Ursolic Acid in Sambucus chinensis Lindl. — Research Paper | ScholarLens