Content Comparison of Ursolic Acid and Oleanolic Acid in Eriobotrya japonica and the Residues of Decoction

The ursolic acid and oleanolic acid from Eriobotrya japonica( Thunb.) Lindl. and the residues of decoction were extracted with theultrasonic technology. A determination method of ursolic acid and oleanolic acid was established with the high performance liquid chromatographand photodiode arraydetector. The chromatographic column, Nova-Pak C18(3.9×300 mm, 4 μm), methanol-water (88∶12, V/V) mobile phase with0.8 mL/min flowrate, the detected wavelength (210 nm), and the column temperature (25 ℃)were adopted. The calibration curve of ursolic acidwas linear under the content of 2.28￣15.96 μg and the correlation coefficient were 0.999 99. The calibration curve of oleanolic acid was linearunder the content of 1.18￣8.26 μg and the correlation coefficient were over 0.999 97. The average recovery of ursolic acid was 99.6 % and RSDwas 1.3 %(n=5). The average recovery of oleanolic acid was 98.5 % and RSD was 1.9 %(n=5). The result showed that the content of ursolic acidand oleanolic acid in the residues in the decoction was higher than in the material. The method was simple, accurate and had wider linear range.The method can be adapted in the evaluation ofthe qualityofmedicinal plants that contained ursolic acid and oleanolic acid.