Effect of angiotensinII on CD40 signaling system in human monocyte cells

Objective:To investigate the influence of angiotensin Ⅱ(AngⅡ) on CD40 signaling system in THP-1 monocyte cell line and macrophages.Methods:THP-1 monocyte cells were cultured in 1640 medium and induced to macrophages by phorbol myristate acetate(PMA).Then the macrophages were cultured in medium containing AngⅡ(10-10～10-5mol/L) and antagonist losartan,PD123319 for 24h.CD40 mRNA level was analyzed by real-time Taqman polymerase chain reaction(PCR).And supernatant CD40 level was examined by enzyme-linked immunosorbent assay.Results:THP-1 monocyte cells were induced to macrophages by PMA(50ng/ml) after 4h.After exposed to varied concentrations of AngⅡ(10-10～10-5mol/L)for 24h,expressions of CD40 mRNA in AngⅡ10-6mol/L group and 10-5mol/L group significantly increased compared with control group(P0.05 both);AT1 antagonist losartan(25μg/ml) inhibited the expression of CD40 mRNA whereas AT2 antagonist PD123319(25μg/ml) showed significant opposite effect(P0.05).After exposed to AngⅡ and its antagonists,CD40 protein levels of supernatant of macrophages showed results parallel to those of CD40 mRNA levels.Conclusion:Angiotensin Ⅱ enhanced the expression of CD40 signalling system in macrophages;two kinds of AT receptor antagonists showed opposite effects on CD40 system.It indicates some relationship between renin-angiotensin-system and CD40 signalling system.