Angiotensin II-induced matrix metalloproteinase-9 expression mediated by NF-κB pathway in human THP-1 cells

AIM:The present study was undertaken to investigate the effect of angiotensin II (AngⅡ) on expression of MMP-9 in THP-1 macrophages. METHODS:Macrophages converted from THP-1 monocytes by incubating with PMA (0.1 μmol/L) for 48 h were divided into PMA group; PMA+AngⅡ group (10-7mol/L,1 h); PMA+AngⅡ+PDTC group (10 μmol/L,30 min) and PDTC group. Western blotting was used to detect the MMP-9 and phosphorylation of NF-κB p65,and the expression of MMP-9 mRNA in THP-1 macrophages was measured by RT-PCR.RESULTS:Compared to control group,the expression of MMP-9 (1.06±0.11,P0.05) and phosphorylation of NF-κB p65 (1.02±0.10,P0.05) in THP-1 macrophages were expressed when treated with AngⅡ (10-7mol/L); and the expression of MMP-9 mRNA were upregulated (1.22±0.08,P0.05). However,NF-κB inhibitor PDTC reduced the NF-κB p65 (0.99±0.12,P0.01) and MMP-9 (1.04±0.14,P0.01) expressions and decreased the expression of MMP-9 mRNA (0.90±0.06,P0.01). CONCLUSION:NF-κB signaling pathway contributes to the expression of MMP-9 in THP-1 macrophage induced by AngⅡ.