Effect of Angiotensin II and Angiotensin-(1-7) at Different Concentrations on Peroxisome Proliferator Activated Receptorγ Expression in THP-1 Macrophage

Aim To investigate the effect of angiotensinⅡ(AngⅡ),angiotensin-(1-7)(Ang(1-7)) at different concentrations on the expression of peroxisome proliferator activated receptorγ(PPARγ) in cultured human THP-1 macrophages. Methods Monocytic THP-1 cells were cultured with 100 nmol/L phorbol myristate acetate(PMA) for 48 hours to lead cells into THP-1 macrophage.Handle the cells in different conditions for 24 hours: medium added nothing,different concentration of Ang-(1-7)(0.1,1,10 μmol/L),different concentration of AngⅡ(0.1,1,5,10 μmol/L).mRNA and protein expression of PPARγ in THP-1 macrophages was measured by RT-PCR and Western Blotting respectively. Results Compared with control group,mRNA and protein expression of PPARγ were increased in Ang(1-7) groups,and they both expressed more as concentration of Ang(1-7) increasing(0.1,1,10 μmol/L).mRNA and protein expressions of PPARγ were decreased in AngⅡgroups compared with control group,and the mRNA and protein both expressed less as concentration of AngⅡ increasing(0.1,1,5,10 μmol/L)(P0.05). Conclusion Ang(1-7) can increase PPARγ expression in THP-1 macrophages in dose-dependent manners.AngⅡcan decrease PPARγ expression in dose-dependent manners.