Protective effect of Yinxingdamo injection on lower limb skeletal muscle ischemia reperfusion in rats

Objective To investigate the effect of yin xingdamo injection on superoxide dismutase,lactatedehpdrogesase and glutataldehyde in rats with lower limb sketetal muscle ischemia reperfusion injury,and light microscope changes of skeletal muscle.Methods The experimental models of lower limb ischemia reperfusion injury were established in 36 Wistar healthy adult rats that were random divided into control group and experimental and treatment group,every group have 12rats.The level of MDA,SOD and LDH were measured respectively in the plasma.The change of issue structure of gastrocnemius muscle were observed with light microscope in every group also.Results The content of SOD in experimental group were significantly lower than that of the control group.But the content of SOD in treatment group was obviously higher than that of the experimental group.And the MDA and LDH levels of experimental group in the plasma were significantly higher than that of the control group.But the MDA and LDH levels of treatment group in the plasma was obviously lower than that of experimental group.In the light microscope examination,the skeletal muscle injury of experimental group was of a severe degree than that of the control group and the skeletal muscle injury of treatment group was a milder degree than that of experimental group.Conclusion Yinxingdamo injection might have protective effect on lower limb skeletal muscle in rats.