Study on protective effects of SOD-model coordination compound MSODa in skeletal muscle ischemia reperfusion injury at rats

Objective To explore the protective effects of SOD-model coordination compound MSODa in skeletal muscle ischemia / reperfusion and its mechanism. Methods The model of hind limb ischemia / reperfusion injury at rats was set up. Ninety-six rats were divided into 4 groups: normal control group (Group Ⅰ),ischemia / reperfusion injury group (Group Ⅱ), normal saline group (Group Ⅲ), MSODa group (Group Ⅳ).The changes of MDA in the plasma ,MPO in the skeletal muscle, CD11b/CD18 on the leukocyte , ICAM-1 in skeletal muscle and the histological changes were studied at 1h, 2h, 4h,8h,12h during reperfusion after ischemia for 4 hours at group Ⅱ, Ⅲ, Ⅳ, respectively. Results Compared to the control group, the expression of MDA, MPO, CD11b/CD18, ICAM-1 were increased evidently in groupⅡ at different intervals. The longer time of reperfusion ,the more serious the injury of skeletal muscle was. In group Ⅳ, the above-mentioned changes were depressed markedly. Conclusions MSODa can inhibit the expression of free radicals and adhesion molecules,consequently resulting in relief of skeletal muscle ischemia-reperfusion injury.