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Determination of Content of Paeoniflorin in Radix Paeoniae Alba by HPLC

LI Ting-li

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Abstract

Objective To compare the quality of different Radix Raeoniae Alba with HPLC methods.Methods The separation was carried out on Hypersil C18 column(4.6 mm×200 mm,5μm);the mobile phase was acetonitrile-H2O(acidified to 0.05% with phosphoric acid)(14∶86);the flow-rate was 1.0mml/min;the column temperature was 25℃.Results There was the highest content of paeoniflorin in Radix Paeoniae Alba of two-year-growing time harvested in October with short storage time.Conclusion The method is simple,rapid with good reproducibility.

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What this paper is about

Objective To compare the quality of different Radix Raeoniae Alba with HPLC methods.Methods The separation was carried out on Hypersil C18 column(4.6 mm×200 mm,5μm);the mobile phase was acetonitrile-H2O(acidified to 0.05% with phosphoric acid)(14∶86);the flow-rate was 1.0mml/min;the column temperature was 25℃.Results There was the highest content of paeoniflorin in Radix Paeoniae Alba of two-year-growing time harvested in October with short storage time.Conclusion The method is simple,rapid with good reproducibility.

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Available abstract

Objective To compare the quality of different Radix Raeoniae Alba with HPLC methods.Methods The separation was carried out on Hypersil C18 column(4.6 mm×200 mm,5μm);the mobile phase was acetonitrile-H2O(acidified to 0.05% with phosphoric acid)(14∶86);the flow-rate was 1.0mml/min;the column temperature was 25℃.Results There was the highest content of paeoniflorin in Radix Paeoniae Alba of two-year-growing time harvested in October with short storage time.Conclusion The method is simple,rapid with good reproducibility.

Key concepts: Paeoniflorin, Chromatography, Radix (gastropod), Phosphoric acid, High-performance liquid chromatography, Chemistry, Content determination, Biology

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