Study on Quality of Radix Paeoniae Alba of Different Origin and Different Growth Stages
越峰 李
Abstract
越峰 李
Abstract
采用HPLC法测定不同白芍药材中芍药苷含量,确定哪种白芍含芍药苷最高。方法:采用色谱柱:Hypersil C18柱(4.6 × 200 mm,5 μm);流动相:乙腈/0.05%磷酸水(14:86);检测:UV 230 nm;流速:1.0 ml/min;柱温:25℃。结果表明:2年生、10月份采收贮存期短的亳白芍芍药苷含量最高。该方法简便,准确可靠,重复性好。 To compare the quality on different Radix Paeoniae Alba using HPLC methods. Column: Hypersil C18 column (4.6 × 200 mm, 5 μm); mobile phase: acetonitrile-H2O (acidified to 0.05% with phosphoric acid) (14:86); UV test: 230 nm; flow-rate: 1.0 ml/min; column temperature: 25˚C. There was the highest content of paeoniflorin in Radix Paeoniae Alba of two-year-growing time, harvest time in October and short storage time from Bozhou. The assay methods are simple, rapid and with good reproducibility.
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采用HPLC法测定不同白芍药材中芍药苷含量,确定哪种白芍含芍药苷最高。方法:采用色谱柱:Hypersil C18柱(4.6 × 200 mm,5 μm);流动相:乙腈/0.05%磷酸水(14:86);检测:UV 230 nm;流速:1.0 ml/min;柱温:25℃。结果表明:2年生、10月份采收贮存期短的亳白芍芍药苷含量最高。该方法简便,准确可靠,重复性好。 To compare the quality on different Radix Paeoniae Alba using HPLC methods. Column: Hypersil C18 column (4.6 × 200 mm, 5 μm); mobile phase: acetonitrile-H2O (acidified to 0.05% with phosphoric acid) (14:86); UV test: 230 nm; flow-rate: 1.0 ml/min; column temperature: 25˚C. There was the highest content of paeoniflorin in Radix Paeoniae Alba of two-year-growing time, harvest time in October and short storage time from Bozhou. The assay methods are simple, rapid and with good reproducibility.
Key concepts: Radix (gastropod), Traditional medicine, Chemistry, Biology, Medicine, Botany