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HPLC Fingerprinting of Radix Paeoniae Alba

Ren Yan-dong

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Abstract

Objective To establish a sensitive and specific HPLC method for controlling the quality of Radix Paeoniae Alba.Methods HPLC method was applied for quality assessment of Radix Paeoniae Alba.Column:Hypersil C18 column(4.6 mm×200 mm,5μm);flow-rate:1.0 ml/min;column temperature:25℃;mobile phase:acetonitrile(A)and H2O(acidified to 0.05% with phosphoric acid)(B).The concentrations of solvent A were 95%,86%,86%and 65%at 0,15,25,60 min,respectively.Results The HPLC chromatographic fingerprint of Radix Paeoniae Alba,showing 14 characteristic peaks,was established from 21 lots of Radix Paeoniae Alba products.The results of similarity analysis was the same as that of herarchical cluster analysis.Conclusion The chromatographic fingerprint of Radix Paeoniae Alba with high specificity can be used to control its quality and assure to lot consistency.

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What this paper is about

Objective To establish a sensitive and specific HPLC method for controlling the quality of Radix Paeoniae Alba.Methods HPLC method was applied for quality assessment of Radix Paeoniae Alba.Column:Hypersil C18 column(4.6 mm×200 mm,5μm);flow-rate:1.0 ml/min;column temperature:25℃;mobile phase:acetonitrile(A)and H2O(acidified to 0.05% with phosphoric acid)(B).The concentrations of solvent A were 95%,86%,86%and 65%at 0,15,25,60 min,respectively.Results The HPLC chromatographic fingerprint of Radix Paeoniae Alba,showing 14 characteristic peaks,was established from 21 lots of Radix Paeoniae Alba products.The results of similarity analysis was the same as that of herarchical cluster analysis.Conclusion The chromatographic fingerprint of Radix Paeoniae Alba with high specificity can be used to control its quality and assure to lot consistency.

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Available abstract

Objective To establish a sensitive and specific HPLC method for controlling the quality of Radix Paeoniae Alba.Methods HPLC method was applied for quality assessment of Radix Paeoniae Alba.Column:Hypersil C18 column(4.6 mm×200 mm,5μm);flow-rate:1.0 ml/min;column temperature:25℃;mobile phase:acetonitrile(A)and H2O(acidified to 0.05% with phosphoric acid)(B).The concentrations of solvent A were 95%,86%,86%and 65%at 0,15,25,60 min,respectively.Results The HPLC chromatographic fingerprint of Radix Paeoniae Alba,showing 14 characteristic peaks,was established from 21 lots of Radix Paeoniae Alba products.The results of similarity analysis was the same as that of herarchical cluster analysis.Conclusion The chromatographic fingerprint of Radix Paeoniae Alba with high specificity can be used to control its quality and assure to lot consistency.

Key concepts: Chromatography, Radix (gastropod), High-performance liquid chromatography, Phosphoric acid, Chemistry, Fingerprint (computing), Biology, Botany

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