Influence of Curcumine on Fas Ligand gene expression and invasive ability of human colon cancer cells

Objective To study the influence of Curcumine on Fas Ligand mRNA expression and invasive ability of human colon cancer cells line SW480.Methods The inhibition concentration 50%(IC50) of Curcumine on human colon cancer cells line SW480 was detected with MTT assay.Affer treated with different concentrations(0.5 IC50,IC50) of Curcumine,the changes of Fas Ligand mRNA expression were detected by the reverse transcription polymerase chain reaction method(RT-PCR)and invasive ability of SW480 cells was measured by Transwell-ECM.Results RT-PCR method revealed that the higher concentration Curcumine upregulated Fas Ligand mRNA expression on SW480 cells(P0.05);Transwell-ECM showed that the higher concentration Curcumine increased apoptosis rate of lymphocyte induced by SW480 cells(P0.05).The comparisons with different concentrations teams of Curcumine had statistical significance(P0.05).Conclusion Curcumine can upregulate functional Fas Ligand mRNA expression of human colon cancer cells line SW480 in some time.At the same time,the upregulative action can raise invasive ability of cancer cells.