Induction of anther and identification of cell chromosome ploidy of alfalfa

The anther of alfalfa was cultured under different conditions of medium,growth regulator formulas and temperatures.The results indicated that low temperature pretreatment for 24 to 48 hours was better than that of treatment for 72 hours for inducing callus.NB medium supplemented with 2,4-D(0.5 mg/L),NAA(0.2 mg/L),6-BA(0.5 mg/L) and KT(3 mg/L) was the best for induction of callus.MS + NAA(0.2 mg/L)+6-BA(3.0 mg/L) was the best for regeneration culture.The higher level of sucrose was suitable for the induction of haploid callus,while the lower level could reduce the browning frequency.