2005•Annual economic survey .../Annual economic survey - Central Bank of Trinidad and TobagoRequires access
Study on Induction Technology of Anther Callus and Seedlings of Alfalfa.
Lifang Wu
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Abstract
At different stages and in different low temperature preliminary treatment and heat stock treatment,the anther of alfalfa was cultured in various medium,different ratio of growth regulator and culture condition.The results indicated that anther selected in September was better than in July and August.Low temperature preliminary treatment for 24~48h was better than that of treatment for 72h for inducing callus.Frequency of inducing callus was higher by combining low temperature preliminary treatment with heat stock treatment.NB medium supplemented with 2,4-D 0.5mg/L,NAA 0.2mg/L,6-BA 0.5 mg/Land KT 3mg/L was optimal for induction of callus.Ratio of growth regulator such as 2,4-D 0.5~2.0mg/L,NAA0.1~1.0mg/L,6-BA0.5~1.0mg/L and KT1.0~3.0mg/L can induce callus.Although the induction rate was low by combinating dark with light,percentage of plantlet was higher.