Cloning and Expression Pattern Analysis of a Promoter of Calmodulin Binding Protein Gene in Arabidopsis

In this study,a promoter fragment of the SCBP60g gene was amplified by PCR from Arabidopsis,and fused with the GUS reporter gene.The recombination construct was then transformed into wild type Arabidopsis.Histochemical staining of GUS activity showed that:the promoter could drive the GUS expression in root,stem,leaf and flower,and relatively high expression levels were found in vascular tissue.However,this expression pattern is different from the promoter of LCBP60g that drives the same GUS gene expression,indicating the specific regulation of this promoter.