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Studies on Genomic DNA Extraction of Cool-season Turf Grass with Improved CTAB Method

Xu Xing

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Abstract

Genome DNA was extracted by the improved CTAB method from Cool-season turf grass and the quality and concentration of DNA were detected.The results indicated that improved CTAB-B method was a simple and high efficient method which could obtain the high quality of genome DNA,the value of OD_260/OD_280 was 1.8 to 1.9,the RNA was eliminated completely without obvious degradation of DNA,the content and purity can meet the requirements of genetic engineering,and digested the results were satisfactory.

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What this paper is about

Genome DNA was extracted by the improved CTAB method from Cool-season turf grass and the quality and concentration of DNA were detected.The results indicated that improved CTAB-B method was a simple and high efficient method which could obtain the high quality of genome DNA,the value of OD_260/OD_280 was 1.8 to 1.9,the RNA was eliminated completely without obvious degradation of DNA,the content and purity can meet the requirements of genetic engineering,and digested the results were satisfactory.

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Available abstract

Genome DNA was extracted by the improved CTAB method from Cool-season turf grass and the quality and concentration of DNA were detected.The results indicated that improved CTAB-B method was a simple and high efficient method which could obtain the high quality of genome DNA,the value of OD_260/OD_280 was 1.8 to 1.9,the RNA was eliminated completely without obvious degradation of DNA,the content and purity can meet the requirements of genetic engineering,and digested the results were satisfactory.

Key concepts: DNA, genomic DNA, DNA extraction, Genome, Extraction (chemistry), Biology, Chemistry, Gene

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