Metastatic Potential Inhibited with Trametes Robiniophila in Highly Metastatic Potential Human Hepatocellular Carcinoma Cells Lines

[Purpose] To explore the mechanism of metastatic potential inhibited with Trametes robiniophila in human hepatocellular carcinoma cells and to provide the experimental basis of Trametes robiniophila in treatment for liver cancer. [Method] Highly metastatic potential human hepatocellular carcinoma cells (MHCC97H) were treated with DMEM and Trametes robiniophila (1mg/ml,3mg/ml,5mg/ml and 10mg/ml) for 24h respectively. The rates of cells adhesion, cell motion and cells invasion were measured. [Results] After MHCC97H cells inculated with Trametes robiniophila for 24h, the rates of cells adhesion decreased and cells motion and cells invasion increased obviously, with significant differences between Trametes robiniophila group and control group. The rates of cells adhesion, cells motion and cells invasion were correlated with concentration of Trametes robiniophila. [Conclusion] Trametes robiniophila markedly inhibit the ability of cells adhesion, motion and invasion in highly metastatic potential human hepatocellular carcinoma, so it can arrest the metastasis of cancer cells.