Research on Apoptosis of Human Hepetocellular Carcinoma Cells Induced by Trametes Rob-iniophila

Objective: We explore the mechanism that Trametes robiniophila inhibits proliferation of the human hepetocellular carcinoma cells and provide the experimental basis for Trametes robiniophila treating liver cancer in clinic. Methods: The human hepetocellular carcinoma cells(MHCC97H)were treated with DMEM,Trametes robiniophila(1mg/ml,3mg/ml,5mg/ml,10mg/ml) and 5-Fu(10ug/ml) for 24h. 48h,72h respectively,and then the rates of apoptosis of the cells were measured in FACSCalibur. Results: The rates of apoptosis of human hepetocellular carcinoma cells in different concentration groups of Trametes robiniophila in same time were higher than that of the control group, there is evident dose dependence in the rise of the rates of apoptosis in every Trametes robiniophila groups,but only the rate of apoptosis in 10mg/ml group corresponds to that of the 5-Fu group. The efficiency that Trametes robiniophila induces apoptosis in the human hepetocellular carcinoma cells amplify with time prolonging. Conclusion: Trametes robiniophila can apparently induce apoptosis in human hepetocellular carcinoma cells,thereby it can inhibit the proliferation of the cells.