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[Purification of EGCG monomer from the extracts of tea by high speed countercurrent chromatography].

Yang Zhang, Yanbin Jiang, Shao-Lie Huang, Ming-Ying Zheng

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Abstract

OBJECTIVE: To investigate the feasibility and techniques for purifying epigallocatechin-3-gallate (EGCG) by high speed countercurrent chromatography (HSCCC). METHODS: A HSCCC unit was used for the purification of EGCG from the extracts of tea. RESULTS: The preferable solvent system was composed of Ether-Ethyl Acetate-H2O (4:10: 25, V/V), and the preferable technical conditions were as follows: the temperature was 35 degrees C, the rotation speed was 800 r/min and the mobile phase flow-rate was 2.5 mL/min. CONCLUSION: The purity of the obtained EGCG sample determined by high performance liquid chromatography (HPLC) is 95%.

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What this paper is about

OBJECTIVE: To investigate the feasibility and techniques for purifying epigallocatechin-3-gallate (EGCG) by high speed countercurrent chromatography (HSCCC). METHODS: A HSCCC unit was used for the purification of EGCG from the extracts of tea. RESULTS: The preferable solvent system was composed of Ether-Ethyl Acetate-H2O (4:10: 25, V/V), and the preferable technical conditions were as follows: the temperature was 35 degrees C, the rotation speed was 800 r/min and the mobile phase flow-rate was 2.5 mL/min. CONCLUSION: The purity of the obtained EGCG sample determined by high performance liquid chromatography (HPLC) is 95%.

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Available abstract

OBJECTIVE: To investigate the feasibility and techniques for purifying epigallocatechin-3-gallate (EGCG) by high speed countercurrent chromatography (HSCCC). METHODS: A HSCCC unit was used for the purification of EGCG from the extracts of tea. RESULTS: The preferable solvent system was composed of Ether-Ethyl Acetate-H2O (4:10: 25, V/V), and the preferable technical conditions were as follows: the temperature was 35 degrees C, the rotation speed was 800 r/min and the mobile phase flow-rate was 2.5 mL/min. CONCLUSION: The purity of the obtained EGCG sample determined by high performance liquid chromatography (HPLC) is 95%.

Key concepts: Countercurrent chromatography, Chromatography, Chemistry, High-performance liquid chromatography, Ethyl acetate, Solvent, Gallate, Organic chemistry

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[Purification of EGCG monomer from the extracts of tea by high speed countercurrent chromatography]. — Research Paper | ScholarLens