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Determination of chlorogenic acid and baicalin in Yinhuang Oral Liquid by HPLC

Qian Jiang

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Abstract

Objective: To establish a method of HPLC for determination of chlorogenic acid and baicalin in Yinhuang Oral Liquid (Flos lonicerae, Radix Scutellariae). Methods: LUNA C18(2) column was used. The acetonitrile 0.1% phosphoric acid solution was used as the mobile phase. The flow rate was 1.0mL·min -1 . The detection wavelength was set as 318nm. Results: For chlorogenic acid, the linear range was 0.2568 ～ 2.0544 μg, and the average recovery rate was 97.7% with RSD =0.8%. For baicalin, the linear range was 0.9896 ～7.9168μg, and the average recovery rate was 98.0% with RSD =0.9%. Conclusion: The method is simple, reliable, accurate and can be applied to the quality control of the preparation.

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What this paper is about

Objective: To establish a method of HPLC for determination of chlorogenic acid and baicalin in Yinhuang Oral Liquid (Flos lonicerae, Radix Scutellariae). Methods: LUNA C18(2) column was used. The acetonitrile 0.1% phosphoric acid solution was used as the mobile phase. The flow rate was 1.0mL·min -1 . The detection wavelength was set as 318nm. Results: For chlorogenic acid, the linear range was 0.2568 ～ 2.0544 μg, and the average recovery rate was 97.7% with RSD =0.8%. For baicalin, the linear range was 0.9896 ～7.9168μg, and the average recovery rate was 98.0% with RSD =0.9%. Conclusion: The method is simple, reliable, accurate and can be applied to the quality control of the preparation.

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Available abstract

Objective: To establish a method of HPLC for determination of chlorogenic acid and baicalin in Yinhuang Oral Liquid (Flos lonicerae, Radix Scutellariae). Methods: LUNA C18(2) column was used. The acetonitrile 0.1% phosphoric acid solution was used as the mobile phase. The flow rate was 1.0mL·min -1 . The detection wavelength was set as 318nm. Results: For chlorogenic acid, the linear range was 0.2568 ～ 2.0544 μg, and the average recovery rate was 97.7% with RSD =0.8%. For baicalin, the linear range was 0.9896 ～7.9168μg, and the average recovery rate was 98.0% with RSD =0.9%. Conclusion: The method is simple, reliable, accurate and can be applied to the quality control of the preparation.

Key concepts: Baicalin, Chlorogenic acid, Phosphoric acid, Chromatography, Flos, High-performance liquid chromatography, Radix (gastropod), Chemistry

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