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2009•The Chinese Journal of Modern Applied PharmacyRequires access

Simultaneously Determination of Chlorogenic Acid and Baicalin in Yinhuang Granules by HPLC

Jun Wang

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Abstract

OBJECTIVE To develop an HPLC method for the simultaneously determination of chlorogenic acid and baicalin in Yinhuang granules.METHODS HPLC was carried out,using Agilent Eclipse XDB-C18(4.6 mm ×150 mm,5 μm) column set at 30 ℃,the acetonitrile-0.1% H3PO4 as mobile phase(using gradient) with flowing rate of 1.0 mL·min-1.The detection wavelength was set at 327 nm.RESULTS The method had good linear relationship within the range of 0.021-2.1 μg(r =0.999 8) for chlorogenic acid and 0.038-3.800 μg(r =0.999 6) for baicalin,respectively.The results of the average recovery were 99.70%(RSD=0.75%) and 99.06%(RSD=1.02%).CONCLUSION The method established was simple and accurate and can be used to simultaneously determine chlorogenic acid and baicalin in Yinhuang granules.

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OBJECTIVE To develop an HPLC method for the simultaneously determination of chlorogenic acid and baicalin in Yinhuang granules.METHODS HPLC was carried out,using Agilent Eclipse XDB-C18(4.6 mm ×150 mm,5 μm) column set at 30 ℃,the acetonitrile-0.1% H3PO4 as mobile phase(using gradient) with flowing rate of 1.0 mL·min-1.The detection wavelength was set at 327 nm.RESULTS The method had good linear relationship within the range of 0.021-2.1 μg(r =0.999 8) for chlorogenic acid and 0.038-3.800 μg(r =0.999 6) for baicalin,respectively.The results of the average recovery were 99.70%(RSD=0.75%) and 99.06%(RSD=1.02%).CONCLUSION The method established was simple and accurate and can be used to simultaneously determine chlorogenic acid and baicalin in Yinhuang granules.

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Available abstract

OBJECTIVE To develop an HPLC method for the simultaneously determination of chlorogenic acid and baicalin in Yinhuang granules.METHODS HPLC was carried out,using Agilent Eclipse XDB-C18(4.6 mm ×150 mm,5 μm) column set at 30 ℃,the acetonitrile-0.1% H3PO4 as mobile phase(using gradient) with flowing rate of 1.0 mL·min-1.The detection wavelength was set at 327 nm.RESULTS The method had good linear relationship within the range of 0.021-2.1 μg(r =0.999 8) for chlorogenic acid and 0.038-3.800 μg(r =0.999 6) for baicalin,respectively.The results of the average recovery were 99.70%(RSD=0.75%) and 99.06%(RSD=1.02%).CONCLUSION The method established was simple and accurate and can be used to simultaneously determine chlorogenic acid and baicalin in Yinhuang granules.

Key concepts: Baicalin, Chlorogenic acid, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Acetonitrile, Detection limit

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