Effect of basic fibroblast growth factor on nerve regeneration of posterior cricoarytenoid muscle in dogs

Objective: To investigate the effects of basic fibroblast growth factor(bFGF) on nerve regeneration of the posterior cricoarytenoid muscles(PCM) in dogs. Methods: After transection of the laryngeal recurrent nerves, the denervated PCM were reinnervated by nerve muscle pedicle implantation in 9 dogs. The animals were divided into 3 groups: bFGF and fibrin glue(FG) group(bFGF+FG), FG group and control group. Functional recovery of PCM was observed laryngoscopically. Electrophysiological acvitity and muscle contract strength were determined. Histochemical studies were demonstrated by Karnovsky and H E stain. Results: Six months after operation, functional recovery and nerve regeneration of the PCA in bFGF+FG group were the best in 3 groups (close to normal), and functional recovery in FG group was better than that in control group. The parameters of electrophysiological acvitity and muscle contract strength were different in bFGF+FG group , FG group, and control group( P 0.05 or P 0.01). Conclusion: bFGF can significantly promote the nerve regeneration of PCA after denervation. FG was surprior to suture in nerve muscle pedicle implantation, and it can cooperate with bFGF in nerve regeneration. [