Expression of stem cell gene Bcl-2 and Oct4 in cervical carcinoma and precancerous tissues and their clinical significance

Objective To investigate the expression of stem cell gene Oct4 and Bcl-2 in cervical cancer and precancerous tissues, and to analyze the clinical significance. Methods HE, immunohistochemistry, RT-PCR and Western blot were used to detect the levels of Oct4 and Bcl-2 protein in normal cervical tissue, and the tissue of cervical intraepithelial neoplasia (CIN Ⅰ, CIN Ⅱ, CIN Ⅲ), cervical carcinoma in situ and invasive carcinoma (well differentiated, moderately differentiated, poorly differentiated). The results were statistically analyzed. Results The expression of Oct4 gradually increased in normal cervical tissue, CIN, and cervical carcinoma, with the expression rates of 20% (2/10), 50% (15/30), 90% (18/20), respectively. The expression rate of Bcl-2 was 10% (1/10) in normal cervical tissue, 40% (12/30) in CIN, and up to 70% (14/20) in cervical carcinoma, which was higher in CIN Ⅲ than CIN Ⅰ and CIN Ⅱ. The expression of Oct4 and Bcl-2 protein showed statistically significant difference between normal cervical tissue, CIN and cervical carcinoma (P0.05), but has nothing to do with the pathological type. Conclusion Oct4 and Bcl-2 may take part in the occurrence and development of cervical carcinoma. Combined detection of Oct4 and Bcl-2 provides useful information for the early diagnosis and treatment of cervical carcinoma.