The Expression and Value of p16INK4A and bcl-2 in Cervical Intraepithelial Neoplasia and Cervical Squamous Carcinoma

Objective To study the expression and value of p16INK4A and bcl-2 in CIN and cervical squamous carcinoma.Methods 39 cases of CIN,24 cervical invasive squamous carcinoma and 20 normal cervical tissues were stained for p16INK4A and bcl-2 by immunohistochemistry method.Results p16INK4A immunoreactivity was absent in all normal cervical tissues,its positive rate in CINⅠ was very low too,but p16INK4A expressed almost in all of CIN Ⅱ/Ⅲ and invasive carcinoma.Bcl-2 immunoreactivity in normal cervical tissues was only seen in cells of basal layer.Its positive rate in CIN Ⅲ and Ⅰ～Ⅱ stage invasive carcinoma were 53.8%and57.1% respectively,which positive cells were diffuse and its staining intensity was higher than in normal cervical tissues.Its positive rate in Ⅲ～Ⅳ stage invasive carcinoma was striking decreased,which positive cells were focal.Conclusion p16INK4A over expression may be seen as a marker of CIN Ⅱ/Ⅲ and invasive carcinoma which is useful in pathological diagnosis.