Adventitious Shoots Regeneration from Leaves of Soft-seeded Pomegranate(Punica granatum L. cv. Yushizi)

To establish the system of rapid in vitro propagation of pomegranate,stems were used to study the effects of different hormone combination on the multiplication of shoots;the leaves were used to study the effects of different hormone combination on the adventitious shoots inducement,then rooting of shoots were studied.The results showed that the optimum media for multiplication of shoots were MS+6-BA 0.3 mg/L+KT 0.3-0.4 mg/L+NAA 0.1mg/L,the optimum media for adventitious buds inducement were MS+TDZ 0.6 mg/L+NAA 0.2 mg/L,the adventitious buds grew well on the inducement medium added GA0.6 mg/L,the optimum media for rooting were 1/2MS+NAA 0.2 mg/L+IBA 0.2 mg/L+AC 0.5 g/L.