Analysis of Cytosinpeptidemycin by HPLC

A quantitative analysis method for determining cytosinpeptidemycin was established by HPLC via optimization of detecting wavelength,mobile phase composition,flow rate and column temperature.The results indicated that when detecting wavelength was 250 nm,a 4:96 mixture of methanol and water was used as mobile phase,flow rate was 0.7 mL/min,and column temperature was 30 °c,the separation effect for cytosinpeptidemycin and the wave shape were the best,and the preserving time was relatively suitable;there was a good linear relationship within the range of 0.01~2.0 mg/L.Therefore,this method is simple and quick,and the testing result is stable and accurate.