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2006•Journal of Huizhou UniversityRequires access

Comparison of Different Extraction Methods of Genomic DNA from Mangifera Indica L.

Duan Zhong, Zhuang Hai

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Abstract

Genomic DNA of Mangifera indica L. was extracted by means of four different methods of SDS, improved SDS, CTAB and modified CTAB.The extraction DNA quality of these methods has been compared.The result showed that modified CTAB method could get rid of secondary substances such as hydroxybenzene and polysaccharides from the solution, and the DNA sample obtained by this method got the highest purity (OD260/OD280 = 1-89) and concentration (767.2 ng /μl) in the four methods.

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What this paper is about

Genomic DNA of Mangifera indica L. was extracted by means of four different methods of SDS, improved SDS, CTAB and modified CTAB.The extraction DNA quality of these methods has been compared.The result showed that modified CTAB method could get rid of secondary substances such as hydroxybenzene and polysaccharides from the solution, and the DNA sample obtained by this method got the highest purity (OD260/OD280 = 1-89) and concentration (767.2 ng /μl) in the four methods.

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Available abstract

Genomic DNA of Mangifera indica L. was extracted by means of four different methods of SDS, improved SDS, CTAB and modified CTAB.The extraction DNA quality of these methods has been compared.The result showed that modified CTAB method could get rid of secondary substances such as hydroxybenzene and polysaccharides from the solution, and the DNA sample obtained by this method got the highest purity (OD260/OD280 = 1-89) and concentration (767.2 ng /μl) in the four methods.

Key concepts: Mangifera, genomic DNA, Extraction (chemistry), Chromatography, DNA extraction, DNA, Chemistry, Polysaccharide

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