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2011•Unpublished venueRequires access

Determination of icariin in Erxianzengjing mixture by HPLC

KE Zun-qiong

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Abstract

Objective To establish a HPLC method for the determination of icariin in Erxianzengjing mixture.Methods The HPLC column was sinochrom ODS-bp(4.6 mm×250 mm,5 μm) column.The mobile phase was consisted of acetonitrile-water(26∶74)at the flow rate of 0.9 mL·min-1.The column temperature was 30 ℃,and the detecting wavelengt was 269 nm.Results Icariin was linear in the concentration range of 0.118-1-1.18 mg·mL-1(r=0.999 4),and the average recovery was 99.03%(RSD=0.63%,n=9).Conclusion This method is simple,accurate,reproducible and can be used for the quality control of this preparation.

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What this paper is about

Objective To establish a HPLC method for the determination of icariin in Erxianzengjing mixture.Methods The HPLC column was sinochrom ODS-bp(4.6 mm×250 mm,5 μm) column.The mobile phase was consisted of acetonitrile-water(26∶74)at the flow rate of 0.9 mL·min-1.The column temperature was 30 ℃,and the detecting wavelengt was 269 nm.Results Icariin was linear in the concentration range of 0.118-1-1.18 mg·mL-1(r=0.999 4),and the average recovery was 99.03%(RSD=0.63%,n=9).Conclusion This method is simple,accurate,reproducible and can be used for the quality control of this preparation.

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Available abstract

Objective To establish a HPLC method for the determination of icariin in Erxianzengjing mixture.Methods The HPLC column was sinochrom ODS-bp(4.6 mm×250 mm,5 μm) column.The mobile phase was consisted of acetonitrile-water(26∶74)at the flow rate of 0.9 mL·min-1.The column temperature was 30 ℃,and the detecting wavelengt was 269 nm.Results Icariin was linear in the concentration range of 0.118-1-1.18 mg·mL-1(r=0.999 4),and the average recovery was 99.03%(RSD=0.63%,n=9).Conclusion This method is simple,accurate,reproducible and can be used for the quality control of this preparation.

Key concepts: Icariin, Chromatography, Chemistry, High-performance liquid chromatography, Linear range, Acetonitrile, Detection limit, Medicine

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