Extraction and Purification of Alkaloids from Lotus Plumule Powder with Macroporous Resin

In the paper, we extracted the alkaloids from lotus plumule powder by using the ethanol refluxing method, and used column chromatography to separate and purify alkaloids extracts further. The results showed that, D101 macroporous resin was more appropriate for purifying the alkaloids, and the maximum adsorbing capacity 9.55mg/g was achieved with the following conditions: sample concentration 10.0mg/mL, pH value 9, volume 30mL(0.43 BV) and flow rate 1mL/min.The best elution conditions were as follows: the ethanol concentration was 70%, with the pH value of 2, volume of 4 BV and flow rate of 1.4mL/min, and the desorption ratio was up to 89.93%, while the final yield of the purified alkaloids was64.39%.