DEVELOPMENT OF BIOCHEMICAL COMPOUND DISINFECTANT AND OBSERVATION OF ITS DISINFECTION-RELATED PROPERTIES

Objective To study the new biochemical compound disinfectant composed of chitosan,amino-acid salt kinase activin enzyme of halobios and polyhexamethylene guanidine hydrochloride,and to observe its disinfection effect.Methods Carrier immersion quantitative germicidal test,physicochemical analysis and animal experiment were used to test germicidal efficacy,physicochemical properties and toxicity of the disinfectant.Results Its main germicidal ingredient were 500 mg/L chitosan,500 mg/L polyhexamethylene guanidine hydrochloride,and certain concentration of ethanol.The disinfectant containing 300 mg/L polyhexamethylene guanidine hydrochloride with a 2 minutes contact time killed 99.99% of Escherichia coli,Staphylococcus aureus and Candida albicans on carrier.After the stock solution of the disifectant was stored at 37℃ for 90 d,its germicidal efficacy not decreased.50% calf serum in bacterial suspension had no obvious influence on its germicidal efficacy.Its oral LD50 for mice was 5000 mg/kg,and cumulative toxicity test showed weak cumulative toxicity.Both the mouse bone marrow polychromatic erythrocyte micronucleus test and the sperm malformation test were negative.The score of stock solution of this disinfectant in test for rabbit eye irritation was 3.Score of skin acute irritation test was 0.Conclusion The new biochemical compound disinfectant has good germicidal efficacy,good stability,no toxicity,minor irritation to eye,no mutagenicity and no teratogenicity.