The expression and significance of PTEN and hMSH2 in the development of cervical carcinoma

Objective To explore the expression of tumor suppressor gene PTEN and human mismatch repair gene hMSH2 in cervical carcinoma and the relationship between them.Methods Immunohistochemistry was applied to detect the expression of PTEN and hMSH2 in normal cervical tissues(34 cases),cervical intraepithelial neoplasia(CINI,CINII,CINIII,101 cases)and cervical carcinoma(46 cases).Results The expression of PTEN protein decreased gradually in normal cervical tissues,CIN and cervical carcinoma.The expression of PTEN protein in cervical carcinoma group was significantly lower than that in other groups(P0.01).Compared between CIN and normal cervical tissue,there was no significant difference(P0.05).The expression of PTEN protein in CINIII was significantly lower than CINI(P0.05).The expression of hMSH2 protein increased in normal cervical tissues,CIN and cervical carcinoma group gradually,compared with other groups,the expression of hMSH2 protein in cervical carcinoma group was significantly higher(P0.01),compared between CIN and normal cervical group there were significant differences(P0.01),the expression in CINII group was higher than CINI group(P0.05).Based on Spearman correlation analysis,there were negative correlation in cervical carcinoma,both the expression of PTEN and hMSH2 protein(P0.05).Conclusion PTEN and hMSH2 protein may play important roles in the development of cervical carcinoma.It may be helpful for predicting and diagnosing cervical carcinoma in early stage.