Effects of Exogenous ABA on the Seed Germination of Rice (Oryza sativa L.) and the Expression of Relative Genes

Many important aspects during plant development are regulated by abscisic acid (ABA).In this study,the seeds from rice variety “Jiayu 948”(Oryza sativa L.) were germinated in ABA solution using three kind methods of ABA treatments.The morphological characteristics of the seeding growth were observed.The results showed that exogenous ABA had affects on the frequency of seed germination and inhibited the growth rate of rice seed obviously (1A3A and 1N3A),but the effects of the inhibition were partially reversible (1A3N).Low concentration of ABA could induce the root growth slightly (1A3N).Furthermore,the expression patterns of three genes were analyzed,O.sativa Mitogen -activated protein kinase 5 (OsMAPK5),β-glucosidase2 (BGLU2) and Oryza sativa Casein kinaseⅠ1 (OsCKI1) during the germination of rice seeds(1A3A).We found that the expressions of OsMAPK5,OsCKI1 and BGLU2 were down-regulated along with the increasing concentration of ABA and changed in root and shoot.