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2005•Chinese Journal of Hospital PharmacyRequires access

Determination of the content of chlorogenic acid in Yinqiao Shangfeng capsules by HPLC

Zhang Li-ping

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Abstract

OBJECTIVE To establish a HPLC method for determination of the content of chlorogenic acid in Yinqiao Shangfeng capsules. METHODS SHIMADZU Shim-packVP-ODS column was used and the mobile phase consisted of methanol and water and acetic acid (20∶80∶0.8) .It was detected at 327 nm.RESULTS The linear range of chlorogenic acid was 0.114 5-0.722 7 μg,r=0.999 8. The average recovery was 96.31%,RSD was 0.77%.CONCLUSION The method is simple,and accurate with a good reprodu cibility and can be used as quantitative analysis method for this preparation.

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What this paper is about

OBJECTIVE To establish a HPLC method for determination of the content of chlorogenic acid in Yinqiao Shangfeng capsules. METHODS SHIMADZU Shim-packVP-ODS column was used and the mobile phase consisted of methanol and water and acetic acid (20∶80∶0.8) .It was detected at 327 nm.RESULTS The linear range of chlorogenic acid was 0.114 5-0.722 7 μg,r=0.999 8. The average recovery was 96.31%,RSD was 0.77%.CONCLUSION The method is simple,and accurate with a good reprodu cibility and can be used as quantitative analysis method for this preparation.

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Available abstract

OBJECTIVE To establish a HPLC method for determination of the content of chlorogenic acid in Yinqiao Shangfeng capsules. METHODS SHIMADZU Shim-packVP-ODS column was used and the mobile phase consisted of methanol and water and acetic acid (20∶80∶0.8) .It was detected at 327 nm.RESULTS The linear range of chlorogenic acid was 0.114 5-0.722 7 μg,r=0.999 8. The average recovery was 96.31%,RSD was 0.77%.CONCLUSION The method is simple,and accurate with a good reprodu cibility and can be used as quantitative analysis method for this preparation.

Key concepts: Chlorogenic acid, Chromatography, Acetic acid, Content determination, Chemistry, High-performance liquid chromatography, Linear range, Methanol

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