2004•Chinese Journal of PharmaceuticalsRequires access

Determination of Chlorogenic Acid in WeiC Yinqiao Granules by HPLC

Ping Huang

Abstract

A HPLC method for determination of chlorogenic acid in WeiC Yinqiao granules was established. A KFC18 column was used with the mobile phase of acetonitrile-0.4% phosphoric acid(10∶90), at the detection wavelengthof 327nm. The flow rate was 1.0ml/min. The calibration curve was linear in the range of 0.4～8mg (r=0.9999). Theaverage recovery was 99.5%, with RSD of 0.81%.

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Key concepts: Chemistry, Chlorogenic acid, Calibration curve, Chromatography, Phosphoric acid, High-performance liquid chromatography, Linear range, Detection limit