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2008•Chinese Journal of Drug Application and MonitoringRequires access

Determination of chlorogenic acid in yinqiao gankang capsules by high performance liquid chromatography

Ling Li

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Abstract

Objective: To establish a HPLC method for the determination of chlorogenic acid in yinqiao gankang capsules. Methods: Chlorogenic acid was determined by HPLC method. A Shim-pack VP - ODS C18 column (250 mm × 4.6 mm, 5 μm) was used and the mobile phase consisted of methanol-water-acetic acid-triethylamine(15 ∶ 85 ∶ 1 ∶ 0.3)at a flow rate of 1.0 mL·min-1. The UV- detection wavelength was set on 324 nm. Results: The linear range of chlorogenic acid was 5.12 ～ 153.60 μg·mL -1 (r = 0.999 9). The average recovery was 98.72%, and the RSD was 0.46%. Conclusion: The method is simple, accurate and stable, and it can be used for the quality control of yinqiao gankang capsules.

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What this paper is about

Objective: To establish a HPLC method for the determination of chlorogenic acid in yinqiao gankang capsules. Methods: Chlorogenic acid was determined by HPLC method. A Shim-pack VP - ODS C18 column (250 mm × 4.6 mm, 5 μm) was used and the mobile phase consisted of methanol-water-acetic acid-triethylamine(15 ∶ 85 ∶ 1 ∶ 0.3)at a flow rate of 1.0 mL·min-1. The UV- detection wavelength was set on 324 nm. Results: The linear range of chlorogenic acid was 5.12 ～ 153.60 μg·mL -1 (r = 0.999 9). The average recovery was 98.72%, and the RSD was 0.46%. Conclusion: The method is simple, accurate and stable, and it can be used for the quality control of yinqiao gankang capsules.

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Available abstract

Objective: To establish a HPLC method for the determination of chlorogenic acid in yinqiao gankang capsules. Methods: Chlorogenic acid was determined by HPLC method. A Shim-pack VP - ODS C18 column (250 mm × 4.6 mm, 5 μm) was used and the mobile phase consisted of methanol-water-acetic acid-triethylamine(15 ∶ 85 ∶ 1 ∶ 0.3)at a flow rate of 1.0 mL·min-1. The UV- detection wavelength was set on 324 nm. Results: The linear range of chlorogenic acid was 5.12 ～ 153.60 μg·mL -1 (r = 0.999 9). The average recovery was 98.72%, and the RSD was 0.46%. Conclusion: The method is simple, accurate and stable, and it can be used for the quality control of yinqiao gankang capsules.

Key concepts: Chlorogenic acid, Chromatography, High-performance liquid chromatography, Triethylamine, Acetic acid, Medicine, Content determination, Chemistry

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